OMICs technologies have become important tools and resources in the pathogenetic investigation of cardiovascular diseases and system medicine. As part of the "DZHKomics" cooperation project, the DZHK provided funds for DNA and RNA sequencing, transport costs for samples and storage media, and data storage for sequence data.
This DNA sequencing project has generated a healthy control resource that will broaden our understanding of how genes contribute to the occurrence of disease.
Application for data
The processed DNA sequencing data of our resource is available to all DZHK members as well as internationally upon request. Requests for use can be submitted on any time. Please submit them to omics.resource(at)dzhk.de using the respective form. The DZHKomics Use and Access Committee will decide on any user query.
- DZHK OR usage applicationAs of october 2018
Current usage projects
|Meder, Haas (Heidelberg)||DZHK OMICs Resources Enable First Association Studies For Genomic Structural Variations In Patients And Controls|
For the generation of this resource, a total of 1,200 whole-genome DNA samples and 600 RNA samples were sequenced. The sample material was selected from six already existing epidemiological cohort studies at various DZHK member institutions:
- Gutenberg-Gesundheitsstudie (GHS) - Universitätsmedizin der Johannes Gutenberg-Universität Mainz
- Hamburg City Health Study (HCHS) - Universitätsklinikum Hamburg-Eppendorf
- HD Supercontrols (HDSC) - Universitätsklinikum Heidelberg
- Kooperative Gesundheitsforschung in der Region Augsburg (KORA) - Helmholtz Zentrum München
- Study of Health in Pomerania (SHIP) - Universitätsmedizin Greifswald
- Instituts für Klinische Molekularbiologie Kiel (IKMB) - Universitätsklinikum Schleswig-Holstein
Sample preparation and quality control
In the run-up to whole-genome and RNA sequencing, SOPs for a harmonic sample preparation were developed specifically for the DZHKomics project. This procedure was intended to minimize potential interferences on the sequencing of samples from different cohorts and to achieve a high comparability of the generated sequence data.
Sequencing. processing, data management
DNA samples were sequenced on HiSeq X machines in the 'High Throughput Sequencing Unit' at the German Cancer Research Center (DKFZ) located in Heidelberg. Data were analyzed at the Institute of Human Genetics (Helmholtz Zentrum München), the Institute for Cardiogenetics (Universität zu Lübeck), and the Institute of Medical Biometry and Statistics (Universität zu Lübeck).
In addition, both the RNA sequencing on the HiSeq Sequencers and the processing of the data were performed at the Max Delbrück Center for Molecular Medicine in Berlin (MDC) .
If you have any questions, please contact the project coordinators Prof. Tanja Zeller, Prof. Jeanette Erdmann and Prof. Heribert Schunkert at omics.resource(at)dzhk.de.