Functional investigationof mutations in cardiovascular disease


Funding ID

81Z1700104

Project number

444

Institution
Universität zu Lübeck
Project leader
Frank Kaiser
Site
Hamburg/Kiel/Lübeck
Short description

To characterize genetic variants associated with cardiovascular disease two different analytic pipelines are used. The first one is focusing on variants affecting protein coding regions … 

To characterize genetic variants associated with cardiovascular disease two different analytic pipelines are used. The first one is focusing on variants affecting protein coding regions of the genome by a broad spectrum of different technologies (two hybrid assays, immunoprecipitation or ChIP) to investigate exchanges of selected amino acids on protein complex formation or protein DNA interaction. The second part of this project is focusing on variants within the non-coding part of the genome. Based on public or own databases variants are rated and listed regarding their predicted functional relevance. Selected variants will be analyzed by cellular in-vitro systems and chromatin conformation capture assays to investigate their effect on chromatin structure and gene expression.

Project type
Partner Site Projects
Keywords
chromatin, SNP, transcription, mutation
Topic
genetics of cardiovascular diseases
Funding
€ 190.000,00
Begin
01.01.2015
End
31.12.2018